Part:BBa_K2220022:Design

Secrete alpha-tubulin protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 286
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 244
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1711
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 685
  Illegal BsaI site found at 1693
  Illegal BsaI.rc site found at 1391
  Illegal SapI site found at 549
  

Design Notes

We have done the codon optimization.

Source

Alpha-factor comes from pyes2/ct/alpha-factor plasmid. Alpha-tubuin http://www.ncbi.nlm.nih.gov/nuccore/393715088